Books

Antibacterial Properties of Endophytic Bacteria Isolated from Kalimantan’s Kratom (Mitragyna speciosa)

---

As the ongoing battle against the escalating challenge of antibiotic resistance persists, pursuing
alternative reservoirs for antimicrobial agents becomes crucial. Endophytic bacteria have surfaced as
promising contenders for novel antibacterial compounds. Due to the increasing popularity of kratom
(Mitragyna speciosa), the Indonesian government encourages the study of potential pharmaceutical
properties of the plant. Therefore, this research aimed to explore the antibacterial activity of 17
isolated endophytic bacteria from the leaf and stem of kratom against common bacteria. This is an
initial effort to identify potential bacterial candidates that can produce bioactive compounds capable
of inhibiting the growth of antibiotic-resistant bacteria. Among the 17 endophytic bacteria, two
coded as KB6 and KB9 exhibited antibacterial activity. However, KB6 extract possessed higher
antibacterial activity against Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923), as
demonstrated by greater inhibition zones, lower minimum inhibitory concentration, and minimum
bactericidal concentration. Further GC-MS analysis showed that the KB6 crude extract contained 50
compounds with numerous identities. Interestingly, several compounds, including mesitylene,
previously reported as a potential antibacterial compound, were also detected. Molecular studies and
microscopy technique confirmed the identity of KB6 as belonging to the genus Bacillus. The
bacterium's ability to produce antibacterial compounds was also confirmed by the presence of the
polyketide synthase type I gene, which is crucial in the biosynthetic pathways of secondary
metabolites. The results of this study implied the potential of endophytic bacteria from kratom as a
reservoir of antibacterial compounds, encouraging a broader scope in antibiotic research.

---

Availability

Detail Information

Series Title

-

Call Number

BT 24-026

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Antibacterial
Bacillus sp
Kratom
Endophytes
PKS type I

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment