Thesis

Anti-cancer Effect Of Laportea Decumana Leaf Extract Towards A549 Cells (non-small Cell Lung Cancer)

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Non-Small Cell Lung Cancer (NSCLC) is a prevalent and highly deadly form of cancer
that affects a significant number of individuals worldwide. The rise in the number of smokers has
correspondingly led to an increase in the incidence of NSCLC. Furthermore, even if smokers
were to quit, it would not lead to a reduction in the severity of NSCLC. A549 is an immortalized
cell derived from NSCLC and was often utilized in biomedicine research. Prior research has
demonstrated the presence of antibacterial properties in L.decumana, and more recent findings
in HT29 and HeLa cell lines suggests that it may potentially exhibit anti-cancer properties by
inducing cancer cells apoptosis. Hence, our project is to investigate the impact of daun gatal
extract on the migration and apoptosis of A549 cells through gene expression analysis of
Vimentin, Fibronectin, E-Cadherin, BAX, and BCL-2 on L.decumana extract treated A549 cells
as well as migration and proliferation assay. Prior to gene expression analysis, the most optimal
concentration of L.decumana extract used for the gene expression analysis was determined
through MTS assay. The migration and proliferation assay indicates that lower concentrations of
L.decumana extract induces migration of A549 cells and higher concentration of L.decumana
extract induces cell death in A549 cells. The gene expression analysis result shows that
L.decumana extract upregulates Vimentin, Firbonectin, E-Cadherin, BAX, and BCL-2
expression. This result thus indicates that L.decumana have potential to be an effective
anti-cancer treatment option under the right dosage.

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Availability

Detail Information

Series Title

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Call Number

BM 24-016

Publisher

i3L, Jakarta : i3L Press., 2025

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

NSCLC
Migration
A549 cells
L.decumana

Specific Detail Info

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Statement of Responsibility

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