Culture of animal cells : a manual of basic technique and specialized applications

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This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:

An all-new section addressing the additional caution required once a primary culture is converted to a cell line
Differentiation in stem cells and reprogramming of adult cells to form stem cells
A new section and protocol on cryopreservation by vitrification
Separate sections dealing with epithelial cells, mesenchymal cells, neurectodermal cells, and hematopoietic cells, a new chapter on germs cells/stemcells/amniocytes
A new section on organotypic culture
Increased emphasis on contamination and misidentification (including cross-contamination) with revised protocols for detection and elimination
A problem-solving chapter, glossary of terms, advice on sourcing materials, and list of suppliers

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Availability

Detail Information

Series Title

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Call Number

571.6381 Fre c

Publisher

West Sussex : Wiley-Blackwell., 2010

Collation

xxxi, 732 p. : ill. : ind. ; 29 cm.

Language

English

ISBN/ISSN

9780470528129

Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

Sixth

Subject(s)

Tissue culture--Laboratory manuals.
Cell culture--Laboratory manuals.
Cells, Cultured.
Culture Techniques--methods.

Specific Detail Info

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Statement of Responsibility

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