In Vitro Investigation of Cinnamon-Ethanol Extract Anti-Adipogenic Effect on …

Obesity, a longstanding global food security issue, has increased in Indonesia since the COVID-19 pandemic due to higher consumption of high-fat, high-sugar foods and more sedentary activity during quarantine. Prevention of obesity can be achieved from the molecular phase through treatment with spice extract, like cinnamon, to mouse 3T3-L1 preadipocytes. The bioactive compound present in ci…

The Effect of Blanching Pretreatment of Cabinet-Dried Javanese Turmeric (Curc…

Hepatocellular carcinoma (HCC) is a primary liver cancer where its development and onset can be caused by oxidative stress. Consequently, consuming Javanese Turmeric as an antioxidant can compensate for the increase of oxidative stress and can be used as a potential liver cancer treatment. However, during the manufacture of Javanese Turmeric as a herbal medicine or supplement, it can alter …

In Vitro Study of Sunscreen Product Towards UV-B and Protection Through Measu…

Ultraviolet light is a type of light that is exhibited from the sun. Ultraviolet is divided into 3 types, including UV-A, UV-B, and UV-C light. These Ultraviolet light might cause several adverse effects including skin and eye damage. Hence, the application of sunscreen is important to prevent the damage induced by UV exposure. Sunscreen is divided into several types including cream, stick, …

Single-cell Analysis of Long Non-coding RNAs (lncRNAs) in Tumor-Associated Ma…

Glioblastoma (GBM) is the most severe type of brain tumor with poor prognosis and low survival rate, characterized by molecular heterogeneity and immunosuppressive microenvironment which pose a significant challenge in improving treatment strategies. It is found that the abundance of tumor- associated macrophages (TAMs) in the GBM microenvironment contributes significantly to tumor progre…

Strategic Bussiness Plan of Wynnergy Drink as Functional Beverage For ModernW…

Earthcell is a brand that is planned to be established as a forward-thinking company and aims to align with Sustainable Development Goals specifically for responsible consumption and production (SDG 12), climate action (SDG 13), good health and well-being (SDG 3). The current market offerings are mostly loaded with products that incorporate artificial ingredients, high doses of caffeine, an…

Research and Development at PT Phapros TBK

PT Phapros TBK is a pharmaceutical company founded in 1954, which is now owned and operated under PT Kimia Farma TBK. Research and development falls under the production department, which is one of the 3 main departments in PT Phapros. The Research and development team centers on drug development and analysis, among which Analytical development plays an integral part in the process. During this…

Validation Of Predicted Sars-cov-2 Nucleocapsid Protein Peptides (np104-122, …

This study investigates the potential of T-cell-mediated immunity in combating SARS-CoV-2, given the concerns about the high mutation rate in the virus's S protein and its potential impact on vaccine efficacy. The prevalent HLA class II alleles in the Indonesian population, namely HLA-DRB1*12:02, HLA-DRB1*15:02, and HLA-DRB1*07:01, play a crucial role in presenting viral peptide antigens to the…

Stards Knockdown Reveals DLC3 Involvement in Vascular Generation and Immune C…

The Deleted in Liver Cancer family houses the RhoGAP proteins DLC1 and DLC2, which have already been known for their tumor suppressive capabilities, and DLC3, whose studies regarding this gene are minimal. This study seeks to elucidate the function of DLC3 protein towards tumor development by looking at the changes in angiogenesis and cell migration rate following DLC3 downregulation, using zeb…

Scalable CD45 and PD-1 Gene Knockout Protocols in T cell using CRISPR/Cas9 Sy…

Chimeric Antigen Receptor (CAR) T cell therapy has revolutionised immunotherapy by boosting T celldriven tumour cell destruction through specific antigen recognition. Despite its success in haematological cancers, significant hurdles in using CAR T cells for solid tumours are the suppression of CAR T cell proliferation and effector functions. This research addresses the pressing challenge in ca…

Immunogenicity Validation Of The Predicted Hla Class Ii T-cell Epitope from S…

The emergence of SARS-CoV-2 variants is primarily attributed to mutations in the S protein, diminishing the efficacy of existing vaccines that aim to generate antibodies against this S protein. As antibodies become less efficient, T cell-mediated immune response becomes essential in eliminating the virus. Three predominant HLA Class II alleles in the Indonesian population; HLA-DRB1*12:02, HLA-D…

Oncolytic Virus Treatment Strategies Against Liver Cancer

Hepatocellular carcinoma (HCC) poses a significant challenge in clinical management due to late-stage diagnosis and limited curative interventions. The imperative to develop more efficacious therapeutic approaches for HCC underscores the exploration of oncolytic virotherapy, an innovative treatment modality exhibiting promising antitumor properties. Among potential oncolytic vectors, the measle…

Identification Of Human Tankyrase 1 Inhibitors As Potential Anti-cancer Agent…

Tankyrase 1 has received attention as a promising target for anti-cancer therapies due to its upregulated expression in various cancer types. Its crucial role in PARsylation leads to the degradation of tumor suppressors (e.g. Axin, TRF1, and PTEN) through the ubiquitin-proteasome pathway, highlighting its significance in oncogenic processes. The urgency to discover novel tankyrase 1 inhibitors …

Novel High Throughput Drug Screening Reveals Therapeutic Opportunities for Pe…

Atypical teratoid rhabdoid tumor (ATRT) is an aggressive pediatric CNS tumor yet lacking standardized therapies while current management standards are not of significant clinical benefit. With the opportunity of accessing the Childhood Cancer Model Atlas (CCMA)’s vast pediatric cancer resources, high-throughput drug screening (HTS) was carried out on the CCMA’s ATRT cohort against the novel…

The Enhancing Effect Of Drug Da-22 On The Immune Response Of Human Neutrophils

Neutrophils are polymorphonuclear and phagocytic leukocytes that act as the first line of innate immune defense to protect and rapidly kill the invading pathogens from the human body. In general, neutrophils are essential immune cells that play a significant role in the early response to infections through their ability to migrate into affected tissues upon pathogens invasion. The activation of…

Effect Of Soluble Dietary Fiber On Micelle Stability During In-vitro Digestio…

Cardiovascular diseases (CVDs) are mainly caused by postprandial lipemia (PPL), an increase of triglycerides-rich lipoprotein level in blood between time after a meal. Food consumption containing dietary fiber has been reported to lower blood triglycerides accumulation. Hence, this study aims to understand the effect of soluble non-viscous dietary fiber towards the lipid absorption in the food …

Synthesis towards Kusunokinin for Anti-Cancer Agent Department of Chemistry, …

This report summarizes a total of 2 months of internship project at the Faculty of Science, Mahidol University, Thailand. This internship project takes place in the science department more specifically in the organic chemistry laboratory. During this internship, the authors tried to synthesize and investigate the intermediates of kusunokinin to enhance its anticancer activity. Kusunokinin inter…

Mechanotransduction Signaling in Abdominal Aortic Aneurysm: The Role of Endot…

The alteration of mechanical and frictional forces composed of pulsatile and shear stress in the aorta is postulated to play a significant role in the abdominal aortic Aneurysm (AAA) pathogenesis. Accordingly, cumulative evidence was gathered to investigate the mechanicalcues, which were believed to have a role in triggering a cascade of signaling events during the shear-induced AAA development…

The Synthesis of Kusunokinin Intermediate for Anti-cancer Agent

Mahidol University is one of the best Campuses in Thailand, which focused on science and medicine. Organic chemistry is part of the department that mainly focuses on the synthesis of natural compounds as well as finding new synthetic methods that are more effective. In this internship oppurtunity, the author got a chance to experience how to work as a chemist. Specifically, this internship proj…

EFFECT OF ADDING METHYLCELLULOSE, KAPPA CARRAGEENAN, AND XANTHAN GUM ON THE P…

People all around the world are shifting towards eating more plant-based food, the vegan consumers and communities are rapidly increasing. However, there is a challenge in the development of plant-based meat analogue to mimic the characteristics of animal based products, especially for the juiciness and textural properties. Hydrocolloids can be used to improve the quality attributes of plant-ba…

Effect of Spray Drying on The Antimicrobial Activity of Different Pediococcus…

Pediococcus acidilactici has gained a lot of interest lately due to its ability as a potential probiotic strain. However, concerns regarding its viability and stability during consumption and storage lead to a solution to utilize spray dry as an encapsulation method for Pediococcus acidilactici. Yet, the utilization of spray drying could affect the cell viability and its functional probiotic pr…

Next-Generation Sequencing and Prediction of T-cell Epitopes from Structural …

The COVID-19 global pandemic caused by SARS-CoV-2 has been going on for almost two years and has negatively impacted the global population. The same as other viruses, SARS-CoV-2 is continuously mutating to adapt to the environment, and this may result in negative impacts, such as an increase in virus transmission, virulence, and even resistance to vaccines. Hence, questions regarding whether th…

Southeast Asian Ovalocytosis (sao) Diagnosis Using Microscopy And Molecular D…

Southeast Asian Ovalocytosis (SAO) is a hereditary genetic disorder caused by a 27 bp deletion in the SLC4A1 gene, leading to a disrupted folding at the extracellular face of the SLC4A1 protein in the red blood cell (RBC) membrane. SAO is an autosomal dominant disorder where heterozygous individuals are asymptomatic and homozygous SAO are considered embryonic lethal. SAO can be observed microsc…

The Effect Of Carboxymethyl Cellulose (cmc) Addition On The Physicochemical A…

Powdered drink is any kind of beverage in the form of “dry” or granules that utilizes the drying process in order to extend the shelf life of the products as well as reduce the quality loss of the product during handling, storage, and distribution. Among all types of powdered beverages, the fastest and largest growth is shown by the fruit-based flavored powder drink. As texture and taste pl…

Elucidating The Impact of EBV Infection on Cell Cycle Dynamic

This research aims to analyze the impact of EBV (Epstein-Barr virus) infection on cell cycle phases (G1, S, G2/M) during latent and lytic phases, exploring alterations in cell cycle regulatory proteins. The investigation extends to compare fixation methods' ethanol and paraformaldehyde. The focus lies in comprehending EBV's effect on host cell cycle progression, emphasizing viral reactivatio…

Investigating Isoflavone-adaptation Synthetic Compounds For Treating Skin Inf…

Psoriasis is an autoimmune skin disorder that has impacted the global population from children to adults. Psoriasis is characterized by unusual proliferation of keratinocytes, leading to itchy, bleeding, scaly, and red lesions due to increased immune system activity. Existing topical treatments often result in side effects, emphasizing the necessity for advanced drug discovery in managing psori…

Cellular and molecular immunology tenth edition

Edition

Tenth edition

ISBN/ISSN

9780323757485

Collation

xii, 587 p. : ill. : ind. ; 28 cm.

Series Title

-

Call Number

616.0797 Abb c

An in vitro study of Pogonatum neesii antioxidant properties towards HaCaT cells

Reactive Oxygen Species (ROS) is a natural byproduct that plays many important roles for the body such as cell signaling, cell survival, and cell death. However, the accumulation of ROS can cause damage to the cell that leads to cell death and eventually to aging. To overcome that, various studies look for antioxidant substances, such as phenolic, flavonoids, and alkaloids. As the study of n…

The Adhesion Ability and The Effects of Probiotic Bacteria to The Presence of…

Lactic acid bacteria (LAB), which are mostly gram-positive bacteria, are frequently utilized in the food fermentation processes. In the last decade, LAB has been used in the development of probiotics to promote health and well-being by boosting the immune system and balancing gut microbes. An effective probiotic must have the capacity to adhere to human epithelial cells, and produce antimic…

Prediction of T-Cell and B-Cell Epitopes From Protein Allergen Tropomysin Tha…

hellfish allergy is one of the highest prevalence allergies in the world, accounting for 7.7% of the population including Indonesia. Thus, the development of T cell epitope and B cell epitope based vaccines play an important role in future prospects for alternative treatment for allergy. The vaccine aims to induce the immune system so it will not react towards the allergen though the body al…

The Screening of Natural Autophagy-Inducing Compounds for Anti-Intracellular …

With the increasing concern about finding a different cure to overcome bacterial skin infections, this research aims to investigate the ability of the innate cell ability to decrease intracellular bacteria infection through autophagy. This study explores and decides several potential naturally-derived autophagy-inducing compounds to be applied to Escherichia coli-infected keratinocytes (HaCaT c…

In Vivo Wound Healing and Antimicrobial Properties of Bio-Cellulose with Gree…

Diabetes is one of the most lethal and ninth most common diseases in the world, which is a metabolic disorder that causes a high and unusual blood sugar level due to the inability of the body to utilize sugar. Diabetes can cause multiple complications and damage to the system, one of them is diabetic foot ulcers (DFU). The most advanced treatment nowadays for DFU is by utilizing bio-cellulose, …

Investigation of Ethanol Miscible Calophyllum inophyllum Extracts (EME) Effec…

Topic Dermatitis (AD) is an inflammatory skin disease characterized by skin rashes and lesions. Downregulation or upregulation of various genes that contribute to the pathogenesis of AD makes it a complex disease. The main contributing genes are the skin barrier genes, FLG, IVL, and inflammatory genes, TSLP, TARC, IL-33, IL-25, MDC, and CTACK which are being investigated in this study. The curr…

Restoration of X-ray Irradiation-Induced Intestinal Damage by Gut Microbiota

The gut microbiota determines the intestinal health status of the host by regulating gut epithelial and immune functions. Depletion of the beneficial gut microbiota after radiation exposure contributes to an exaggerated inflammatory response and dysregulation of intestinal epithelial cells. Previous studies demonstrated that administration of beneficial gut microbiota, Lactobacillus acidophilus…

Analysis of Wound Healing & Antibacterial Properties of Keratin-Immersed Bio-…

Diabetes has been categorized as the fifth highest mortality cause worldwide. The diabetic wound has an altered wound-healing mechanism which leads to longer inflammination stages and higher chances of infection. Hence, it causes chronic wounds. Because it is necessary to keep the wound moist and manage exudate, the wound dressing selections is essential in the management of the wound. Bacteria…

Investigation of Antibacterial Activity And Potential Anti-Inflammatory Prope…

Staphylococcus aureus is an opportunistic pathogen that commonly inhabits the human skin and can cause skin infection, especially in individuals with impaired immune systems when skin is damaged. Treatments usually involve the usage of antibiotics. However, antibiotic effectiveness is in danger due to the fast development of resistant microorganisms worldwide. As a result, there is a growing …

The Effect of Notch Signaling In Hindbrain Serotonin Neurons Differentiation …

Serotonin (5-HT) neurons are currently of research interest since these neurons have a crucial role in regulating human homeostasis and psychological states. These neurons are generated from the hindbrain raphe nuclei and are classified into rostral (r1-r3) and caudal (r5-r8) groups. While rostral groups have been successfully developed, only a few functional caudal 5-HT neurons are available i…

Comparative Study of Umbilical Cord-derived Mesenchymal Stem Cells Secretome …

Skin anti-aging is a primary focus in derma cosmetics, targeting the reactive oxidative species (ROS) pathway for developing anti-aging therapies due to its role in the degradation of dermal extracellular matrix (EDM). Collagen type 1 is targeted to combat skin aging due to its role in preventing wrinkles formation and re-epithelization of human keratinocytes (HaCaT). Cell-free treatments have …

The Use of Keratin-Biocellulose Black Tamanu Oil Hydrogels as A Novel Wound D…

Diabetes mellitus is a global health issue that is gaining traction in our healthcare system which leads to impaired wound healing and results in chronic ulcers, these increases the risk of amputation and disease mortality rate by 50-59% in 2020. Therefore, better inventions to treat diabetic ulcers are required. A bio-cellulose hydrogel is a form of wound dressing that can aid in protecting t…

Developing Protein Complexes for Targeted Aggregation on Cancer Cell Surfaces…

Cancer is one of the deadliest diseases worldwide, with metastasis serving as the primary cause of death. While metastasis has been widely studied, a significant amount still remains unexplored, particularly the role of glycosylation. Glycosylation is a fundamental aspect in a variety of biological processes, indicating that slight changes in it can lead to detrimental effects such as cancer, w…

Immunoinformatics Study of the Multi-epitope Mapping to n Formulate Peptide-b…

"Monkeypox (MPXV) is a zoonotic disease caused by infection with the monkeypox virus (Poxviridae family) which was first identified in Democratic Republic of Congo. World Health Organization (WHO) has received increasing reports of monkeypox (Mpox) cases worldwide, and the mortality rate is 3-6%. Until now, fully effective & specific treatment for monkeypox virus infection is still absent.…

The Effect of Primary Drying Temperature on Freeze-dried Mesenchymal Stem Cel…

Mesenchymal stem cells (MSC) are multipotent stromal cells capable of regenerating and differentiating into various tissues. MSCs can secrete over 200 different types of these bioactive molecules called secretomes, that contain chemokines, interleukins, growth factors, and extracellular matrix proteins. However, the bioactive molecules present in these can degrade over time due to temperatu…

The Influence Of Salinity Concentration On Power Generation And Microbial Via…

The deep-sea ecosystem is a largely unexplored environment with unique chemical and physical conditions that impact the organisms living there. Deep-sea sediments contain a diverse population of electroactive bacteria (EABs) that have the potential to generate electricity in microbial fuel cells (MFCs). This study aims to investigate the influence of salinity concentration on bacterial viabi…

The Study of Different Temperatures Effects on Deep-Sea Sediment Microbial Fu…

The increasing global electricity consumption driven by population growth and industrial development necessitates the exploration of environmentally friendly renewable energy sources. Microbial fuel cells (MFCs), more specifically deep-sea sediment microbial fuel cells, have gained significant attention in the bioenergy industry as they can harness bacteria capability to generate electricit…

Antimicrobial Activity Characterization of Pediococcus Acidilactici Cell-Free…

The demand of a food preservative has been increasing due to foodborne diseases due to foodborne pathogens. Moreover, there is also an issue on the health risks caused chemical food preservatives, further increasing demand on a safer food preservative. Recently, Pediococcus acidilactici has been gaining interest as a potential natural food preservative due to its ability to produce pediocin,…

Optimization Of Liquid Chromatography Analycal Method For Quanficaon …

Erythropoietin is a glycoprotein hormone produced by the kidney. Erythropoietin is a hormone that controls the production of erythrocytic progenitors. Since 1989, erythropoietin (EPO) has been used to treat anemia-related diseases. The formulation of the EPO product was altered in 2004. The occurrence of creutzfeldt-jakob disease (CJD) led to the replacement of the stabilizer, human serum a…

Investigation on the Removal of Depth Filter For an Effective Human Recombina…

Erythropoietin is a glycoprotein hormone produced by the kidney, which triggers the formation of red blood cells in the bone marrow. However, due to chronic kidney disease, the erythropoietin hormone is insufficiently produced in anemic conditions. Hence, a therapeutic recombinant human erythropoietin (rhEPO) is the alternative way to overcome chronic anemia. Genetically engineered host cells,…

Antimicrobial Activity Characterization of Pediococcus Acidilactici Cell-Free…

The demand of a food preservative has been increasing due to foodborne diseases due to foodborne pathogens. Moreover, there is also an issue on the health risks caused chemical food preservatives, further increasing demand on a safer food preservative. Recently, Pediococcus acidilactici has been gaining interest as a potential natural food preservative due to its ability to produce pediocin,…

Effect of Spray-drying Towards Pediococcus acidilactici Adherence Ability to …

"Pediococcus acidilactici is a non-pathogenic probiotic bacteria that can improve gut health by adhering to intestinal cells and inhibiting pathogen adherence. To create an easy-to-use product, probiotics are often produced in powdered form using spray drying. However, this drying method can affect the probiotic's viability and functionality. This study examined the impact of spray drying in…

Comparison of Ethyl Cellulose and Zein as Polymers to Deliver Curcumin and Le…

Curcumin (CUR) has been traditionally used as a wound healing agent, thus, is a good wound dressing agent. To further enhance anti-bacterial prevention, levofloxacin (LEVO) is considered as it has high effectiveness towards both gram-positive and gram-negative bacteria. CUR and LEVO are added to ethyl cellulose (ethocel) due to its high stability towards heat, light, and chemicals. Zein is …

In-Vivo Study Of Wound Healing And Antimicrobial Properties Of Biocellulose I…

Chronic diabec wound is a common complicaon in diabetes mellitus which is caused by a prolonged wound-healing process from a persistent inflammaon phase. Thus, proper wound treatment is needed to promote wound healing and protect the wound from infecon. Biocellulose (BC) as wound dressing provides a moist environment to protect skin and pr…