A marine bacteria Streptomyces BLH 3-7 from Bitung Sea Sulawesi were isolated and observed to produce agarase enzymes in basic ISP2 media. To improve the agarase production, different carbon, nitrogen, pH, and minerals were compared to observe their effects on the agarase production through one factor at a time (OFAT) method. By selecting the best factor one at a time, the optimized media…