This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The protocols are accompanied by extensive discussion of potential pitfalls and troubleshooting advice.
Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathwa…
The book is very well presented, written in a logical and concise way, and unequivocally solid in terms of scientific content, with many ready-to-use, robust procedures tested and validated over the years by recognized experts. I warmly recommend this volume to anyone who wants to implement proteomics techniques in the laboratory, or who simply wishes to get a practical sense of these technique…