Existing protocols for extracting Immunoglobulin G (IgG) from blood spots (DBS) were compared, utilizing fresh, one-week-old, and one-month-old DBS made from a healthy Thai’s blood as the samples. In addition, DBS samples from healthy uninfected villagers obtained from a Malaria survey in 2017 will also be used. To validate the previously published elution protocols and buffers, alteration…