Existing protocols for extracting Immunoglobulin G (IgG) from blood spots (DBS) were compared, utilizing fresh, one-week-old, and one-month-old DBS made from a healthy Thai’s blood as the samples. In addition, DBS samples from healthy uninfected villagers obtained from a Malaria survey in 2017 will also be used. To validate the previously published elution protocols and buffers, alteration…
This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. …