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Isolation of a Prebiotic-Producing Gene (1-SST) in Dioscorea esculenta (Gembi…
Inulin is a group of heterogeneous polysaccharides which is used in food industry as a soluble-fiber prebiotic with an estimate global market value reaching USD 1.5 million by 2023. Further, the low-calorie and gut health-enhancing properties increase the demand for Inulin. In order to meet the demand in Indonesia, the conventional extraction method from plants that are not available in Indones…
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- BT 19-007
Isolation, Characterization, and Identification of Microorganism from …
Soy sauce is a popular food condiment in Asia that is made of soybean, wheat, and salt through a two-step fermentation process. The first step is a solid-state fermentation to produce koji by adding mold spores, typically Aspergillus species, to the mixture of soybean and wheat. The second stage is a brine fermentation or moromi, which is done by immersing …
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- BT 19-006
Isolating 1-SST Gene of Dioscorea esculenta (Gembili) using Primers Designed …
Inulin is a commercial dietary fiber which is commonly extracted from chicory roots. Its demand in food and beverage industry is currently increasing, thus production using biotechnological approach is needed. There are two main fructosyltransferases that take part in its biosynthesis: sucrose:sucrose 1-fructosyltransferase (1-SST) which initiates the conversion of sucrose into 1-kestose and fr…
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- BT 19-005
Constructing and Cloning of PEGFP-N1 Vector Containing Turncated RPGRIP1L Coi…
Ciliopathy is a general term used to describe broad spectrum of diseases caused by primary cilia dysfunction. Different kinds of ciliary gene have been detected to be the underlying causes of this detrimental disease. One of the identified genes is Retinitis Pigmentosa GTPase Regulator 1 Like (RPGRIP1L), which encode transition zone (TZ) protein of the primary cilia. Mutation of this gene has b…
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- BT 19-003
Construction of Plasmid Vector for the Expression of LipYΔPE from Mycobacter…
LipY is a gene product from Mycobacterium tuberculosis that plays an important role in the hydrolysis of triacylglycerol (TAG), especially during the dormant stage. LipY is one of the PE family protein which are involved in immune pathogenicity and virulence of mycobacteria. The PE domain of LipY participates in the modulation of LipY activity. LipY protein devoid of its PE-domain (LipYΔPE) e…
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- BT 19-002
Constructing and Cloning of Pegfp-N1 Vector Containing Turncated RPGRIP1L C2 …
Primary cilia is an antenna like organelle that functions as signal transmitter in eukaryotic cells. RPGRIP1L is one of the protein that is located on the primary cilia, which plays an important role in controlling cellular signaling, assembling the ciliary base, and controlling autophagy. RPGRIP1L is composed of 3 main domains, namely Coiled-coil, C2, and RIDL domain. Among those domains of RP…
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- BT 19-001
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