Thesis
The Effect of Different Storage Temperatures on Micronutrient Stability, Physicochemical Properties, and Shelf Life Estimation of Fortified Cookies
Micronutrient deficiency is a major concern as it affects 50% of pregnant women in the world.
Introduction to food fortification as the nutritional intervention program may aid in the prevention of
micronutrient deficiencies, yet to accomplish the program's goals, the amount of nutrients fortified in
the product must be sufficient and fulfilling. However, exposure to a variety of environmental
conditions throughout storage may lose chemical and physical properties, leading to a change in the
food's properties and nutrition. This study aimed to analyze the effect of different storage
temperatures on the stability of micronutrients, physicochemical properties, and the shelf life
estimation of fortified cookies. The fortified cookies were subjected to different storage temperatures
of 30, 37, and 45°C for 1 month with all analyses done before and after; while shelf life was carried
out every 6 days. The study showed that different storage temperatures significantly decreased the
texture up to 156 ± 42.33 g, which coincided with a significantly increased up to 5.57 ± 0.10 % and
0.62 ± 0.03 in the moisture content and water activity, respectively. A slight fluctuation happened
owing to crystalline sugar on the cookie surface. Iron results with 90-96% retentions presented that it
did not appear to be influenced significantly by the storage temperatures due to its characteristics
that have high stability. Moreover, through the Arrhenius equation, the estimated shelf life of fortified
cookies stored at storage temperatures of 30, 37, and 45°C was 26 days, 12 days, and 6 days,
respectively.
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