Internship Report

The Antioxidant Activity of Litsea oppositifolia Stem Extract Against Oxidative Models on 3T3 Fibroblast Cell Line

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Skin aging is the accumulation of cellular waste that progressively leads to a loss of structural
integrity and physiological function due to intrinsic and extrinsic factors. Although intrinsic aging is
inevitable, it is highly influenced by extrinsic factors, including UV radiation, tobacco smoking and
pollution. These repeated exposure causes the upper dermis fibroblasts to selectively lose through
oxidative damage caused by ROS which gradually reduces the production of the ECM. A natural
compound found in Litsea oppositifolia stem extract was shown to exert antioxidant activity that can
eliminate ROS. Hence, this study was done to evaluate the antioxidant activity of Litsea oppositifolia
stem extract using 3T3 fibroblast cell line treated with H
2
O
. Litsea oppositifolia stem extract was
obtained from the Pharmacy Department of University of Indonesia in the form of paste. Various
concentrations of the extract, ascorbic acid, and H
2
O
2
2
were prepared and treated to the cells to
evaluate the cytotoxicity effects. The results show that Litsea oppositifolia stem extract was less toxic
compared to ascorbic acid. The antioxidant assay was done afterwards using 50 μM H
-induced
oxidative stress and the extract showed that the concentration of 6.25 ppm displayed the most
optimal protection against H
2
O
. This suggests that at a concentration of 6.25 ppm, Litsea
oppositifolia stem extract would serve as the ideal concentration to be produced as cosmetics.

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Availability

Detail Information

Series Title

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Call Number

BM 22-008

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

-

Carrier Type

-

Edition

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Subject(s)

Antioxidant
Ascorbic acid
ECM
ROS
Litsea oppositifolia
H viii 2 O 2

Specific Detail Info

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Statement of Responsibility

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