Thesis
Effect of Effective Microorganism 4 (EM4) on Fermentation Duration of Eco-Eenzyme Using Citrus Peel Waste (CPW)
Increase in waste production, especially municipal house waste (MSW), has pushed the need for
alternate ways to tackle waste management. One of these methods is to recycle food waste,
fermenting them to form eco-enzyme (EE). One of the requirements to make EE is letting it ferment
for 3 months, which may deter its adoption in household settings. Effective Microorganism 4 (EM4) is
a commercially available inoculant that is promoted to help with decomposition. This study aims to
investigate the effect of EM4 towards accelerating the fermentation process of eco-enzyme. The
substrate that was used to make EE was citrus fruit peels (CPW) from Citrus x sinensis. The EE was
made using a 1:3:10 ratio of molasses, waste, and water. The fermentation time lasted for 70 days.
The parameters that were measured to determine this were; pH, temperature, and enzyme activity.
Results showed that there was a significant difference in the pH progression between EE and eco-
enzyme with EM4 (EEM) (p-value = 0.005). However, no temperature difference was seen.
Furthermore, no enzymatic activity was detected, which is crucial in an eco-enzyme. Hence, it can be
concluded that EM4 did not have any effect in accelerating the fermentation process of eco-enzyme.
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