Thesis
Shelf-Life Estimation of Co-crystallized Red Fruit Oil (Pandanus Conoideus Lam.) Based on Arrhenius Model
Red fruit (Pandanus conoideus Lam.) contains high amounts of carotenoid which contribute to its
distinct red color that makes it a suitable source of natural colorant. Encapsulation through cocrystallization
is conducted to increase applicability of red fruit oil. The research aims to assess the
effect of different storage temperatures on its quality parameters, total carotenoid content, peroxide
value, moisture content and color, assess the degradation kinetics of co-crystallized RFO in terms of
its quality parameters stored in three different temperatures as wells as estimating the shelf life
of co-crystallized RFO using accelerated shelf life testing based on Arrhenius model. Significant
differences in TCC were observed between samples stored at different temperatures, whereas no
significant differences in PV, moisture content and color were observed between samples stored at
different temperatures. TCC degrades with the first-order kinetic reaction, whereas PV and color
degrades with zero-order kinetic reaction. Total carotenoid content has the highest activation energy
of 836.01 J/ mol K, and shelf life of 480 days at 20 °C and 322 days at 25 °C. Followed by peroxide
value with an activation energy of 276 J/mol K and shelf life of 95 days at 20 °C and 83 days at 25° C.
Color has the lowest activation energy and shortest shelf life period of 119.05 J/mol K and shelf life of
10 days at 20°C and 9 days at 25°C. Moisture content does not fulfill the R
2
value requirement for the
Arrhenius model. Estimation of shelf life is based on the quality parameter of PV.
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