Thesis

The Adhesion Ability and The Effects of Probiotic Bacteria to The Presence of Pathogenic Bacteria on Caco-2 Cell

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Lactic acid bacteria (LAB), which are mostly gram-positive bacteria, are frequently utilized in the food
fermentation processes. In the last decade, LAB has been used in the development of probiotics to
promote health and well-being by boosting the immune system and balancing gut microbes. An
effective probiotic must have the capacity to adhere to human epithelial cells, and produce
antimicrobial substances; bacteriocins, while also not bringing any toxic effects to the hosts. Several
surface proteins are necessary for probiotics to adhere to human intestinal cells and by having the
ability, it will prevent the attachment of pathogenic bacteria to the intestinal cells. Enterococcus
faecium and Lactococcus lactis are well-characterized LAB that has suitable properties to be
considered as probiotics and have been widely used in pharmaceutical and food processes. However,
research and clinical trials on the effects of these probiotics on human cell lines are still lacking. This
research was done to investigate the adhesion ability of these LAB on Caco-2 cell lines by using the
adhesion assay and also to observe their safety and toxicity along with the presence of pathogenic
bacteria by using the MTT assay. The results showed that both LAB have good adhesive ability with
73% and 72% for both L. lactis and E. faecium respectively. Moreover, the result also showed that
both LAB have a good protective effect to Caco-2 cells against ETEC and S. typhi. In conclusion, both L.
lactis and E. faecium indicate good probiotic bacteria and might be used to counteract food-borne
pathogens, especially ETEC and S. typhi.

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Availability

Detail Information

Series Title

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Call Number

BM 23-005

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Salmonella typhi
Lactic Acid Bacteria
Enterococcus faecium
Lactococcus lactis
Enterotoxigenic Escherichia coli
Caco-2 cell

Specific Detail Info

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Statement of Responsibility

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