Report

Generation Of Reporter Flaviviruses And Noroviruses For Antiviral Discovery And Evaluation

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This study aims to generate molecular infectious clones, incorporating reporter genes (GFP and
SfCherry), for flaviviruses and noroviruses—namely, Kunjin, Zika, and Murine Norovirus (MNV). The
objective is to use viral constructs for antiviral compound screening and unravel their mechanisms of
action. Employing a diverse array of methods, including PCR, CPER, transfection, fluorescence, and
diagnostic tests like Plaque Assay and Western Blot, the investigation successfully produced
molecular infectious clones for the Kunjin Virus. Regrettably, the construction of molecular infectious
clones for the Zika Virus encountered challenges. Conversely, Murine Norovirus (MNV) demonstrated
success in transfection methods and Western Blot assays but exhibited no conclusive results in the
Plaque Assay. These findings contribute valuable insights into virological studies, presenting both
successes and areas for further exploration in refining antiviral research methodologies.

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Availability

Detail Information

Series Title

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Call Number

EP BM-028

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

EP BM-028

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Flaviviruses
Noroviruses
Molecular Infectious Clones
GFP
SfCherry

Specific Detail Info

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Statement of Responsibility

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Other version/related

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