Thesis

Semi-Quantitative Analysis of Triterpenoid Saponin in Hydroponics Abrus Prcatorius Induced by Methyl Jasmonate

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Abrus precatorius is a plant highly valued for its foods and medicinal benefits, notably due to the
triterpenoid saponins that it can synthesize. The triterpenoid saponins produced are glycyrrhizin in
the roots and abrusoside in the leaves. However, the yield of triterpenoid saponins in A. precatorius is
low. To increase the yield, it can be induced by activating the stress condition for the plant.
Waterlogging through hydroponics can be utilized to cause oxidative stress to the plants. In addition
to that, methyl jasmonate (MeJa) is a hormone for stress signalling in plants. Applying MeJa to a
specific part of the plant can increase the stress signal, triggering the expression of secondary
metabolites, and creating triterpenoid saponins. Therefore, to investigate the impact of hydroponics
and MeJa on triterpenoid saponins levels in A. precatorius, a semi-quantitative analysis of saponins
will be performed through Liebermann-Burchard test and quantified with UV-vis spectrophotometer.
The results showed hydroponics having lower saponin content compared to soil, but MeJa treated
hydroponics showed an increase in saponin content. It is recommended for further research to
analyze using more precise analysis methods such as HPLC or GC to check the effects on specific
saponin found in A. precatorius and conduct antioxidant or betulinic acid analysis to confirm the
non-MeJa treated hydroponics results.

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Availability

Detail Information

Series Title

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Call Number

BT 24-010

Publisher

i3L, Jakarta : ., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

triterpenoids
Abrus precatorius
saponin
Methyl Jasmonate
semi-quantitative analysis

Specific Detail Info

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Statement of Responsibility

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