Thesis

Risk Assessment of Ochratoxin A (OTA) in Indonesia from Robusta Coffee (Coffea canephora) Originated from West Java using Margin of Exposure Approach

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Ochratoxin A (OTA) is a carcinogenic mycotoxin that is widely present in coffee beans and
causes public health concern that can widely grow in Indonesia’s temperature. This present study
aims to evaluate the OTA contamination level in Robusta coffee beans Indonesia using LC-MS/MS
analysis. The age group and consumption rate was determined from the literature as follows: 19-25
years old (highest consumption compared to other age groups) and 30 grams per cup (highest serving
size). Risk assessment of OTA was calculated using EDI (Estimated Daily Intake) and MOE (Margin of
Exposure) Approach. Physicochemical analysis of OTA (water activity, moisture content, and pH) were
analyzed as well. Contamination level of OTA that was found in this study was not detected as it was
below the Limit of Detection (0.886 ppb), so the LOD of 0.886 ppb was used for risk assessment. Even
though it was as low as LOD, the MOE obtained was still in an unsafe range (below 10.000) and had
high risk. Additionally, physicochemical properties have crucial factors to the development of
OTA-produced fungi and several factors that are affecting different ranges of pH, water activity, and
moisture content are roasting time and process. This study suggests lowering the OTA exposure risk
by lowering the consumption rate of coffee into 15 grams per cup, and suggests coffee drinkers
population in Indonesia (25-39 years old) to consume coffee in 3 cups/month for females and 3.5
cups/month for male in order to reduce the OTA risk.

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Availability

Detail Information

Series Title

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Call Number

FT 24-022

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Coffee
, LC-MS
Ochratoxin A (OTA)
Risk Assessment
Robusta

Specific Detail Info

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Statement of Responsibility

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