Pediococcus acidilactici is a gram-positive lactic acid bacteria (LAB) widely used as a probiotic due to its safety, benefits, and ability to survive in the harsh environment of the gastrointestinal (GI) tract. One of the challenges for probiotic species is the presence of bile salt, which can degrade bacterial cell membrane due to its main function in lipid solubilization. Bile salt can als…
Bile salt hydrolase (BSH) is important to help bile salt deconjugation, converting bile salt into a more hydrophobic form which helps to alleviate cholesterol level in the body. BSH is a N-terminal nucleophile superfamily enzyme, and frequently found in lactic acid bacteria (LAB). Spray drying, as one of preservation methods, is often applied for commercial production, and known to reduce th…