Protein Purification of Phytoplasmal Effector Causing Phyllody 1 (PHYL1)

Phytoplasmas, which are bacteria that lack cell walls, are known to infect a variety of host plants and spread quickly within them including fruit plants and other types of flora causing significant agricultural losses. Phytoplasmas infect a wide variety of plants, causing irregular plant growth such as phyllody. Phyllody is a developmental transformation in plants that transforms flowers into …

Protein Purification of Pir A - linker - Pir B

Photorhabdus insect-related toxins A and B (Pir A and Pir B) are two different types of insecticidal toxins that were firstly found in Photorhabdus luminescens. Its homolog, from Vibrio parahaemolyticus, is found to cause acute hepatopancreatic necrosis disease (AHPND) in the shrimp, since it contains plasmid pVA1. When binded together, Pir A and B can exert its effect, because they form a stru…

Expression and Purification of A Bioactive Recombinant Fungal Immunomodulator…

Fungi, particularly medicinal mushrooms, have been extensively harvested for their natural compounds for alternative medicine because they provide benefits with little to no adverse effects. FIPs, or fungal immunomodulatory proteins, are one that have been extensively studied for their immunomodulatory activities to promote lymphocyte production and subsequently enhance cytokine production. FIP…

Investigation on the Removal of Depth Filter For an Effective Human Recombina…

Erythropoietin is a glycoprotein hormone produced by the kidney, which triggers the formation of red blood cells in the bone marrow. However, due to chronic kidney disease, the erythropoietin hormone is insufficiently produced in anemic conditions. Hence, a therapeutic recombinant human erythropoietin (rhEPO) is the alternative way to overcome chronic anemia. Genetically engineered host cells,…

Investigation on the removal of Depth Filter for an effective Human Recombina…

Erythropoietin is a glycoprotein hormone produced by the kidney, which triggers the formation of red blood cells in the bone marrow. However, due to chronic kidney disease, the erythropoietin hormone is insufficiently produced in anemic conditions. Hence, a therapeutic recombinant human erythropoietin (rhEPO) is the alternative way to overcome chronic anemia. Genetically engineered host ce…

Protein Expression and Purification of Taq Polymerase

The development and application of molecular diagnostic technologies has triggered a revolution in infectious disease diagnosis and surveillance over the years. The ability to deliver a quick, specific, reliable, cost-effective, and straightforward alternative is one of the advantages of using molecular diagnostics. Taq polymerase is an example of an enzyme that is required in molecular di…

Bioenergy production by anaerobic digestion : using agricultural biomass and …

Interest in anaerobic digestion (AD), the process of energy production through the production of biogas, has increased rapidly in recent years. Agricultural and other organic waste are important substrates that can be treated by AD. This book is one of the first to provide a broad introduction to anaerobic digestion and its potential to turn agricultural crops or crop residues, animal and o…

Metabolomics tools for natural product discovery : methods and protocols

Classical natural product chemistry is transitioning to modern day metabolomics as a result of the advent of comprehensive analytical platforms and sensitive analytical instrumentation. Therefore, it is worthwhile to summarize recent developments with current analytical platforms and highlight how metabolomics is being integrated into this classical field to dereplicate and profile natural prod…

Purifying proteins for proteomics : a laboratory manual

This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The protocols are accompanied by extensive discussion of potential pitfalls and troubleshooting advice.

Purification of laboratory chemicals

Purification of Laboratory Chemicals keeps engineers, scientists, chemists, biochemists and students up to date with the purification of the chemical reagents with which they work, the processes for their purification, and guides readers on critical safety and hazards for the safe handling of chemicals and processes. The Seventh Edition is fully updated and provides expanded coverage of the lat…

Low cost emergency water purification technologies : integrated water securit…

Natural disasters, tornadoes, hurricanes, and floods are occurring with increasing frequency. In emergencies, pure drinking water is quickly the most important item. Low Cost Emergency Water Purification Technologies provides the tips and techniques for supplying potable drinking water at low cost in the direst circumstances. Succinct and readable, this manual describes the various options f…

Handbook of wastewater treatment : biological methods, technology, and enviro…

Edition

-

ISBN/ISSN

9781622575916

Collation

xiv, 337 p. : ill. : ind. ; 27 cm.

Series Title

-

Call Number

628.35 Han

Basic methods in protein purification and analysis : a laboratory manual

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in…