Arak, a traditional Indonesian spirit typically produced from rice or sugarcane, is made by fermenting rice or fruit juice followed by distillation. This research explores using snake fruit (Salacca Zalacca var Pondoh) as an innovative raw material for Arak production, offering a novel flavor profile and creating a niche market. Snake fruit, containing fermentable sugars, presents a cost-eff…
Diabetes mellitus (DM) is a metabolic disease where insulin production and/or action is defective, leading to a chronic high blood sugar level (hyperglycemia), in which the highest type is type 2 diabetes mellitus (T2DM). Some of the pharmacological treatments are metformin and sitagliptin where the combination treatment of them provides more effects compared to the monotherapy. An imported…
Background. Next Generation Sequencing (NGS) is a technology that possesses tremendous potential in advancing precision medicine in oncology. However, in Indonesia, the progress of its integration remains hampered. This research is conducted with the hope of providing exploratory research for the general NGS maturity in Indonesia, its current level of implementation, and future challenges f…
Personalized medicine helps make decisions about illness prevention, diagnosis, and therapy based on a person's genetic profile. To use personalized medicine, genetic testing using Next generation sequencing (NGS) performed to determine the best drugs to treat the diseases. Cancer can be treated using personalized medicine. Each year, the number of cancer patients is rising, and with person…
Lung cancer, among all types of cancer, leads first in cancer incidence and accounts for the first largest source of cancer-related mortality worldwide in 2022. Such a high incidence and mortality rate is primarily due to the majority of patients being diagnosed with advanced stages of cancer. Chemotherapy remains a primary option for metastasized NSCLC, however, chemotherapy still possesse…
Green supply chain management has been a popular approach for many businesses to respond to many environmental issues, such as climate change, loss of biodiversity, resource depletion, greenhouse emissions, and climate change. This research aims to explore the effects of supply chain management on organizational sustainability performance and how it affects the products produced by the manu…
Photorhabdus insect-related toxins A and B (Pir A and Pir B) are two different types of insecticidal toxins that were firstly found in Photorhabdus luminescens. Its homolog, from Vibrio parahaemolyticus, is found to cause acute hepatopancreatic necrosis disease (AHPND) in the shrimp, since it contains plasmid pVA1. When binded together, Pir A and B can exert its effect, because they form a stru…
The Deleted in Liver Cancer family houses the RhoGAP proteins DLC1 and DLC2, which have already been known for their tumor suppressive capabilities, and DLC3, whose studies regarding this gene are minimal. This study seeks to elucidate the function of DLC3 protein towards tumor development by looking at the changes in angiogenesis and cell migration rate following DLC3 downregulation, using zeb…
This internship program at PT Ramuraga Tirta Lestari aimed to optimize and improve various aspects of kombucha production, including food safety, data organization, and production efficiency. The intern worked on several projects to achieve this goal, such as calibrating pH meters to ensure accurate measurement of acidity levels in kombucha, sterilizing bottles to prevent contamination and main…
An expensive Vanilla planifolia Andrew. Var. Vania 2 is commonly propagated using a stem cutting technique which is associated with low propagation rate. A method of callus regeneration was assessed in this experiment to increase the yield of vanilla plants by utilizing different concentrations and combinations of BAP and NAA supplemented into the MS media. The objectives of this experi…
The COVID-19 global pandemic caused by SARS-CoV-2 has been going on for almost two years and has negatively impacted the global population. The same as other viruses, SARS-CoV-2 is continuously mutating to adapt to the environment, and this may result in negative impacts, such as an increase in virus transmission, virulence, and even resistance to vaccines. Hence, questions regarding whether th…
The author was doing an internship at PT. Alamii Natura Sejahtera as a research and development intern for 6 months starting from July 2023 – January 2024. The author has several responsibilities including doing research for both optimizing existing product production processes as well as new product development. Additionally, the author also helps the team with the document-making for BPOM-r…
The overarching goal of this project is to comprehend the intricate dynamics of RNA viruses, specifically influenza A viruses, enteroviruses, and coronaviruses, which pose significant health threats. The focus lies in exploring hostvirus interactions, particularly the roles of host components in various stages of viral RNA replication, aiming to identify potential targets for antiviral interv…
Axon degeneration played a part in the development of neurodegenerative disease for instance Alzheimer’s diseases, chemotherapy-induced neuropathy, and traumatic brain injury. It is found that axon degeneration is present prior to the onset of symptoms. Therefore, scrutinizing the molecular mechanism is detrimental. Recently, it has been found that SARM1 NADase play a role in the developmenta…
Modified cassava flour (mocaf), is one of the cassava fermentation products using lactic acid bacteria. Mocaf is generally safe for people that have certain conditions due to its gluten-free. It also contains low-protein but rich in carbohydrates. Fermentation starter can affect the quality of mocaf. Pediococcus acidilactici has a potential as a starter for fermented food. Until now there is no…
Folic acid is an important vitamin for fetal development and nucleotide and amino acid metabolism. Corn is one of the good sources for folic acid while tempeh fermentation has been reported to increase folic acid content. To concentrate further, membrane filtration is a promising technology. This study aims to understand the effect of microfiltration conditions towards the increase of folic aci…
Cervical cancer is the fourth most prevalent cancer and is the primary cause of mortality among women on a global scale. It is deadly once metastasize and therefore its early detection and prevention of its metastasis was crucial for the survival of the patient. Treatment targeting the prevention of metastasis was a necessity. Laportea decumana is a plant which has been shown to possess anti-ca…
The deep-sea ecosystem is a largely unexplored environment with unique chemical and physical conditions that impact the organisms living there. Deep-sea sediments contain a diverse population of electroactive bacteria (EABs) that have the potential to generate electricity in microbial fuel cells (MFCs). This study aims to investigate the influence of salinity concentration on bacterial viabi…
The increasing global electricity consumption driven by population growth and industrial development necessitates the exploration of environmentally friendly renewable energy sources. Microbial fuel cells (MFCs), more specifically deep-sea sediment microbial fuel cells, have gained significant attention in the bioenergy industry as they can harness bacteria capability to generate electricit…
Erythropoietin is a glycoprotein hormone produced by the kidney, which triggers the formation of red blood cells in the bone marrow. However, due to chronic kidney disease, the erythropoietin hormone is insufficiently produced in anemic conditions. Hence, a therapeutic recombinant human erythropoietin (rhEPO) is the alternative way to overcome chronic anemia. Genetically engineered host cells,…
Syzygium aromaticum, commonly known as cloves, is considered as one of the richest dietary sources of antioxidant and polyphenolic compounds. The bioactivity and antimicrobial activity of this spice is enhanced by using novel extraction techniques such as ultrasound-assisted extraction (UAE). Sample-to-solvent ratio is one of the important factors that impact the functional properties of pl…
Strawberries are known for their vibrant color, juicy texture, and enticing sweetness, which may change as they ripen. A freeze-drying (FD) process was used to alter the changes. However, because FD is a time-consuming process, a pretreatment process is essential for accelerating the FD process. This research aims to improve the FD process for strawberries by using osmotic dehydration (OD) a…
Food seasoning powder which are used as and/or within food products should have free-flowing properties. Powder caking is one of the adverse effects of seasoning powder with poor free-flowing properties. A solution to prevent powder caking is by improving the composition of the seasoning powder. It can be achieved by substituting the liquid-based to powder-based ingredients and/or utilization o…
Pediococcus acidilactici is a lactic acid bacteria that has been found on many fermented foods around the world, showing its capability as a starter culture. It is gaining research interest due to its probiotic potential that can provide additional health benefits, further increasing its value as starter culture. Starter cultures are typically commercialized in powder form, with one of the …
Erythropoietin is a glycoprotein hormone produced by the kidney, which triggers the formation of red blood cells in the bone marrow. However, due to chronic kidney disease, the erythropoietin hormone is insufficiently produced in anemic conditions. Hence, a therapeutic recombinant human erythropoietin (rhEPO) is the alternative way to overcome chronic anemia. Genetically engineered host ce…
The appropriate course of action to stop the spread of COVID-19 involves immunizations due to the rise in COVID-19 cases. Pichia pastoris serves as the protein expression system for RBD, a spike protein produced by the SARS-CoV-2 virus. RBD is utilized as an antigen for vaccine candidates to tackle COVID-19 which is suitable for low to middle-income countries. The Tangential Flow Filtration…
Mass Drug Administration is one of the strategy use by countries to intervene the progression of helminth infection. The goal of MDA program is to give preventive chemotherapy to a populations by using broad spectrum anti-helminthic drugs. The drugs were administered by the people once or twice a year depending on the prevalence of their region. The Indonesian government has applied this …
The production of pharmaceuticals involves intricate procedures that carry a great deal of risk which can compromise the final product's quality. Moreover, the manufacturing of biologics must adhere to the laws, statutes, regulations, and guidelines. Thus, the Quality Assurance (QA) department is required to ensure and guarantee that the standards required by the consumers and the law are met a…
PT Kalbio Global Medika (KGM) is a subsidiary company of PT Kalbe Farma Tbk. which is a pioneer biopharmaceutical-manufacturing company in the country. The analytical method development (AnDev) team in the research and development (RnD) department of KGM is responsible for verifications of the analytical methods used throughout the manufacturing process in KGM and for analytical studies in …
SARS-CoV-2 began its outbreak in the late December of 2019 and was later declared as a global pandemic. It has led to the research of therapeutics and vaccines development for SARS-CoV-2 as a method of treatment. The target genes for the immunotherapeutic construct are usually the spike protein due to its crucial binding to the human ACE2 receptor via the RBD. Upon its entrance to the host …
Diospyros digyna or also known as Black Sapote is considered as an exotic-climacteric fruit that is harvested when still unripe. However, black sapote consumption rate is still very low as it is deemed as an uncommon fruit.The ripening of black sapote can take up to 10 days, which is deemed as uneconomical in a fast-moving industry. To shorten the ripening period, ripening using calcium carbide…
PT ABC Kogen Dairy is a dairy company that has proven consistency in product innovation throughout the years. The marketing department, where the author had her internship, also contributes to product development projects focusing on the marketing attributes. Two yogurt projects will be discussed in this report, the first one is EPD which was conducted due to the inadequate performance of t…
Chrysanthemum morifolium is an ornamental plant that recently has had an increase in the demand. The in vitro propagation of C. morifolium var. Marimar at UPT Agribisnis Pontianak resulted in the low shoot produced. Therefore, the attempt for increasing the shoot proliferation by the utilization of coconut water (CW) and 6-Benzylaminopurine (BAP) as the growth regulation in ½ MS media was …
Nowadays, the various variants of SARS-CoV-2 have been found along with the time. It has been marked down that the antigenic drift takes a part of those occurrences. In genome mapping, SARS-CoV-2 has the largest Open Reading Frame (ORF) called ORF1ab which translates 16 different Non-Structural Protein (NSP) for viral replication. This project aims to predict the conservancy of the ORF1ab d…
COVID-19 pandemic has negatively governed the world, especially in the healthcare sectors. The continuous influx of patients in the hospital without any definitive treatment has become a major concern for healthcare professionals in handling COVID-19 patients. This research study aims to use absolute lymphocyte count (ALC) and neutrophil-to-lymphocyte ratio (NLR) as a prognostic tool for determ…
Cosmetics have been widely used to improve the skin condition. Niacinamide is known as the vitamin B3 which works to repair the skin condition. Unfortunately, It has a limited ability to penetrate the skin. Therefore, a penetration enhancers is important to be conducted to ensure an adequate activity of the active ingredients. Several types of glycols were tested and expected that the differ…
Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) is a newly emerging virus well known as the major cause of worldwide pandemic due to Coronavirus Disease 2019 (COVID-19). Major breakthroughs in the Next Generation Sequencing (NGS) field were elucidated following the first release of a full length SARS-CoV-2 genome in January 10th 2020, with the hope to turn the table against worseni…