Molecular Detection of Plasmodium falciparum Histidine-rich Protein 2/3 Gene …

Malaria is a major health issue worldwide, with Papua being the epicenter of malaria in Indonesia. To reduce malaria transmission, diagnostic methods have become a crucial pillar in malaria control and elimination. Due to their simplicity, rapid diagnostic tests (RDTs) are used to diagnose malaria in areas lacking expert microscopists, specifically PfHRP2-based RDTs. However, the emergence …

Screening of Isoflavone-Modified Compounds as Potential Therapeutic Agents to…

inflammation plays a crucial role in the pathogenesis of various skin conditions such as psoriasis and atopic dermatitis, primarily driven by abnormal cytokine production. This study investigates the anti-inflammatory potential of several synthetic isoflavone-modified compounds (CYR-6811k, CYR-6812l, CYR-6813m, CYR-6815o, CYR-6816p) by examining their effects on cytokine production in THP-1 …

The Antibacterial Activity of DA-22 through Enhancing the Functions of Human …

Neutrophils are a type of white blood cell that act as the first line of the innate immune system to protect and against invading pathogens from the human body. Generally, neutrophils are the most abundant cells that play an important role in response to infections through their ability to migrate into affected tissue upon pathogens invasion. The activation of neutrophils are able to engage …

Detection and Genomic Characterisation of Dengue Virus (DENV) from Clinical I…

Dengue virus (DENV) infection presents a significant global health threat, especially in Indonesia. This study focuses on identification and genetic characterization of circulating DENV isolates in Timika and Jambi through serotyping, genotyping, and mutation analysis to understand genetic diversity and transmission dynamics. Samples from 119 and 115 suspected dengue patients in Timika and J…

Transcriptional Profiling of Malaria-exposed Mosquitoes: a Comparative Analys…

Malaria, caused by Plasmodium parasites and transmitted by Anopheles mosquitoes, is a significant public health issue, particularly in South and Southeast Asia where Plasmodium vivax is prevalent. Understanding the genetic factors influencing mosquito susceptibility to P. vivax is crucial for developing targeted malaria control and eradication strategies. Building on previous findings that …

Inhibition of MTHFD2 Activity Suppresses Beige Adipogenesis In Vitro

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BM 24-011

Expression Analysis of Cilia-Associated CCDC Genes in Lung Development and Re…

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BM 24-001

Assessing anti-influenza A response and host gene regulation of Daun Gatal (L…

Influenza A virus (IAV) remains a significant global health concern, prompting ongoing efforts to explore novel antiviral agents. This study investigates the potential of Laportea decumana (Daun Gatal) extract as an antiviral against IAV using A549 cell lines. The objective was to evaluate the extract's impact on cell viability, cytotoxicity, and viral replication dynamics. A series of cytot…

Molecular Surveillance Of Antimalarial Drug Resistance Genes In Plasmodium Fa…

Malaria is a disease caused by infection of the Plasmodium parasite that spreads through Anopheles mosquitoes, which mainly plagues humans living in regions with poor healthcare and countries around the equator. Among the five species capable of causing malaria in humans, Plasmodium falciparum and Plasmodium vivax are noted as the most threatening species, as their manifestations in humans …

Investigation of Non-Muscle Myosin II Genes Expression During Lung Developmen…

Lung is vital to the intricate maintenance of oxygen and carbon dioxide exchange which is regulated by specialised cell lineages. This regulation involves tight coordination of epithelial cells regulated by Non-Muscle Myosin II (NM II). Although adult lung cells are typically quiescent, Acute Lung Injury (ALI) may disturb the homeostasis and cause loss of airway epithelial layer. This will c…

eIF2α-ATF4 Signaling Mediates NASH Progression through Suppression of Kupffe…

Nonalcoholic steatohepatitis (NASH) is a complex liver condition marked by hepatic steatosis, injury, inflammation, and fibrosis. It is often triggered by obesity-induced ER stress which activates the integrated stress response (ISR) pathway. This pathway, involving eIF2α and ATF4, contributes to NASH progression, particularly fibrosis via hepatic stellate cells (HSCs) activation. However, …

Pooled Whole-genome CRISPR/Cas12a Loss-of-Function Screens Reveal A Novel Pre…

Though rare, atypical teratoid/rhabdoid tumors (ATRT) remain a clinically challenging pediatric tumor and lack defined treatments. “One-size-fits-all” multimodal regimens have largely been of modest clinical benefits thereby urging the development of precision medicine for ATRT. However, the single- mutation nature of pediatric tumors like ATRT has misrepresented the potential of non-mut…

In Vitro Assessment of The Antibiofilm Properties of Curcuma SP. Essential Oi…

Biofilm formation poses a significant health challenge, playing a role in the pathogenesis of biofilm-associated diseases, including dental caries, a chronic infectious disease with widespread prevalence that significantly impacts oral health. Lactobacillus acidophilus is one of the primary cariogenic microorganisms associated with the progression of dental caries. In order to address this …

The Properties of Daun Dewa as Antiviral Compound

The demand for continuous discovery of novel antimicrobial compounds is still very high, especially considering the pandemic the world has recently faced. Many types of antimicrobials can be found in natural ingredients and the vast biodiversity of Indonesia certainly holds many natural compounds that have the potential to be a new novel antimicrobial compound. Gynura divaricata, or also kn…

Investigation of the kunyit asam herbal drink potential as an alternative tre…

Non-small cell lung cancer (NSCLC), accounting for more than 85% of lung cancer diagnoses, has remained the leading cause of cancer-related death. Chemotherapy has been used as the gold standard treatment to treat it, and one of the common drugs is cisplatin. However, cisplatin can lead to resistance, resulting from a complex interaction of molecular pathways that reduces the efficacy of ci…

Preliminary investigation of glutamine transporter SLC7A8 role in mediating c…

Glioblastoma multiforme (GBM) is a highly aggressive glioma that is reported to exhibit elevated glutamine uptake and metabolism, termed as glutamine addiction, often associated with poor patient prognosis. Recently, a preliminary study identified glutamine transporter SLC7A8 to be highly upregulated in resistant GBM and associated with poor prognosis, with results intriguingly showing dist…

Investigating The Role Of JNK In Multi-drug Resistant ER+ Breast Cancer

Endocrine therapy and CDK4/6 inhibition are the standard-of-care treatments for patients with advanced or metastatic estrogen receptor-positive (ER+) breast cancer. Despite improvements in the overall progression-free survival, resistance develops in patients by unidentified mechanisms. Preliminary CRISPR/Cas9 screens from the Caldon lab revealed that loss of the MAPK9 gene (encoding JNK2) …

Optimization of the Protocol for Human Immunoglobulin G (IgG) Extraction from…

Existing protocols for extracting Immunoglobulin G (IgG) from blood spots (DBS) were compared, utilizing fresh, one-week-old, and one-month-old DBS made from a healthy Thai’s blood as the samples. In addition, DBS samples from healthy uninfected villagers obtained from a Malaria survey in 2017 will also be used. To validate the previously published elution protocols and buffers, alteration…

Investigating the Interaction Between RAD54 Terminal Domains to RAD54B Motor …

DNA damage is a complex phenomenon arising from both endogenous and exogenous sources, posing threats to genomic stability and necessitating an effective DNA damage response (DDR). A dysfunctional DDR can cause genomic instability, which is defined by a higher frequency of DNA mutations. Genomic instability is a common feature of many cancers, and it can result in oncogenic mutations and tu…

Investigation of Anti-Cancer Potentials between Astragaloside IV and Querceti…

Colorectal cancer is the third place cause of death tumor globally. And its frequent prevalence may be caused by the conventional treatments that has been applied. One of the conventional or commonly used treatments are chemotherapy, one of colorectal cancer common chemotherapy are oxaliplatin. This would create drug-resistant cancer. For this effort in counteracting the resistant caused by …

Immunogenicity Validation of Predicted HLA Class II-binding Peptides Derived …

SARS-CoV-2, the cause of the COVID-19 pandemic, prompted rapid vaccine development. However, emerging variants challenge antibody-mediated protection. Targeting T-cell immunity is promising due to its crucial role in eliminating infected cells and the difficulty of viral evasion via HLA diversity. Despite this potential, there is a lack of HLA studies and SARS-CoV-2 epitope mapping in the I…

Exploring the Impact of Andaliman (Zanthoxylum acanthopodium) Fruit Crude Ext…

This study investigates the anti-inflammatory properties of Andaliman (Zanthoxylum acanthopodium DC.) fruit extract on inflamed human keratinocytes (HaCaT cells). The phytochemical profile of Andaliman reveals a high content of terpenoids from the Salkowski test, which inhibits the NF-κB signaling pathway, thereby suppressing pro-inflammatory cytokine production. The study demonstrates a s…

The Therapeutic Potential of DX23 in Elevating Neutrophil Antimicrobial Defense

Neutrophils play an important role in the innate immune mechanism. During pathogen encounter, these cells gather at the infection site and perform various functions. Neutrophil could effectively eliminate pathogens by performing their functions such as respiratory burst, degranulation, phagocytosis, and formation of neutrophil extracellular traps (NETs). Therefore, neutrophil activation eme…

Evaluating the Role of Gene X in Pancreatic Adenocarcinoma (PAAD) Survivabili…

Pancreatic adenocarcinoma (PAAD) remains a life-threatening disease with the most unfavorable prognosis among all cancer types. This is mainly due to the high rate of cell survivability marked by rapid cell proliferation and migration activity. Despite having KRAS oncogene as a known commonly mutated driver gene, targeting it has been challenging and has hindered the development of effective…

Investigation of the Antifungal and Anti-inflammatory Properties of Alpinian …

Candida albicans is a yeast that resides on the human skin, however it can become an opportunistic pathogen when the immune system is compromised or there are imbalances in the microenvironment. This microorganism can cause candidiasis which is able to cause high mortality rates in especially immunocompromised patients. Treating this infection is highly difficult due to similarities in stru…

"Construction of Mini-genome System for both Rabies virus Laboratory Strain a…

Rabies virus (RABV) causes a zoonotic disease known as rabies that affects humans and mammals. With a genome length of 12 kb, RABV is classified as a non-segmented, negative-stranded RNA virus that encodes five different structural proteins. The nucleoprotein (N), phosphoprotein (P), and large RNA polymerase protein (L) forms ribonucleoprotein (RNP); together with the viral genome it serves …

Investigating the Role of HDXX as a Novel DNA Damage Response Protein in Colo…

Colorectal cancer (CRC) ranks third in cancer incidence and cancer-related mortalities worldwide. The transformation of normal colon and rectal cells to malignant adenocarcinomas due to unrepaired DNA damage which leads to oncogenic mutations. These mutations also increase the frequency of DNA damage, leading to endogenous replication stress (RS). This introduces an opportunity to induce sy…

The Utilization of Next Generation Sequencing in Analyzing HIV Whole Genome

Human Immunodeficiency Virus (HIV) is a retrovirus responsible for causing acquired immunodeficiency syndrome (AIDS). The pol gene of the HIV genome encodes protease and reverse transcriptase, and integrase region which is responsible for viral infection and survival inside the host cell. Antiretroviral Therapy is usually administered to target these regions due to their significance in vir…

Immunoinformatics Study of Potential Molecular Mimicry between Viral T-Cell E…

There have been reports regarding the autoimmunity induced by SARS-CoV-2 in COVID-19 patients, such as: GBS, SLE, multiple sclerosis, demyelinating neuropathies, and orthostatic tachycardia syndrome, narcolepsy, even vaccine-induced. However, the exact mechanism is still unclear. One of possible reason is molecular mimicry, a condition in which the T-cell epitopes come from the virus antige…

IFM is a Potential Key Player in AML Cell Survival and CML Cell Resistance to…

Myeloid leukemia is characterized by the excessive proliferation of myeloid cells within the bone marrow. It is categorized into acute (AML) and chronic (CML) myeloid leukemia, with distinct etiologies and therapeutic complexities. The genetic heterogeneity of AML contributes to varying treatment responses, leading to a high mortality rate. One gene that has been identified as an adverse pro…

In vitro Investigation on The Anti-adherence and Antibacterial Effect of Lact…

Humans have been co-existing with a whole population of microbes, where trillions of different microbes are living inside the human GI tracts as a commensal gut microbiota. This condition provides a mutual relationship where the gut microbiota serves as a natural protection for the intestinal tract homeostasis. Nevertheless, the human intestinal tract is constantly exposed to environmental …

In Silico Design and ELONA Validation of an Aptamer for SARS- CoV-2 Omicron R…

COVID-19, caused by the SARS-CoV-2 virus, has resulted in a pandemic with 6.8 million confirmed cases in Indonesia as of December 2023. The high number of cases has necessitated the need for robust and specific diagnostic tools. Despite its effectiveness, the qRT-PCR suggested by the World Health Organization has a number of drawbacks, such as expensive costs, the need for trained personnel …

Investigation on Effect of Cell Seeding Number Toward Hepatic Multicellular T…

Cancer remains a global health challenge in need of precise, cost-effective, and scalable research tools for comprehensive investigations. Spheroid-based methodologies emerge as reliable, easily adaptable, and highly scalable techniques, offering an improved representation of in-vivo conditions compared to conventional 2D cell cultures. This study utilizes the Huh-7 cell line to generate hep…

Electrophysiological Evaluation of the Left-Right Asymmetry of MHb-IPN Pathwa…

The Medial Habenula-Interpeduncular Nucleus (MHb-IPN) pathway is an emerging with recently discovered roles in fear memory expression. As ongoing research revealed the asymmetric functions of this pathway in mammals, questions were asked regarding the developmental origins of this asymmetry, particularly its relation to the visceral asymmetry. This study set out to investigate the lateral a…

Red Fruit Oil Co-Crystal Development as a Natural Food Colorant by Utilizing …

Red fruit oil (RFO) extract serves as a natural red colorant but exhibits limitations related to solubility and susceptibility to environmental factors such as oxygen, heat, and light-induced degradation. Encapsulation through co-crystallization represents a promising approach to mitigate these challenges. In this context, erythritol is employed instead of sucrose during co-crystallization t…

The Product Development of Alcohol-Free Craft Beer from Thai Bitter Melons (M…

This research aims to develop an alcohol free craft beer utilizing Momordica charantia, otherwise known as bitter melon, as a hops substitute. This study substitutes hops with 0%, 25%, 50%, 75%, and 100% of bitter melon. The alcohol free beer was prepared by malting, mashing, brewing, fermentation, and incubation, followed by giving heat treatment to the beer separating them into non-heat t…

The Effect of Alpha-Amylase Types and Time of Enzyme Activation Towards the S…

Plant-based or non-dairy milk substitutes are becoming favored in the growing field of functional beverages driven by factors such as health benefits, allergies, lactose intolerance, and the rise in vegan diets. However, there are challenges to producing oat milk, such as achieving the desirable texture and taste. This study is aimed to have 4 treatments utilizing 2 different kinds of amylas…

Comparing Physicochemical Properties Of White Bread Produced Using Natural Ye…

The choice of yeast, whether it's grape yeast or commercial yeast, depends on the baker's objectives and preferences. Grape yeast is ideal for experimental bakers seeking unique flavors, while commercial yeast offers reliability and consistency. In a recent study, the physicochemical properties (water activity, texture, and moisture content) of white bread made from grape yeast and commercia…

Physicochemical and Sensory Properties of Steamed Banana Cake Made From Banan…

Bananas are one of the most popular fruits consumed in Indonesia. It is the fruit that Indonesia produces the most, with 9.2 million tons produced in 2022 and 91 kg consumed weekly per person. Vitamins and minerals are all found in good amounts in bananas. Bananas are a versatile fruit that can be used for various kinds of food, such as banana cake. However, banana cake contains gluten and …

Extraction Optimization of Gotu Kola (Centella asiatica) Leaf using Ultrasoun…

The food technology industry is increasingly interested in innovative ways to include nutritious ingredients that promote health in food products. The leafy green plant that we used as a traditional medicine known as Gotu Kola (Centella asiatica), which is regional to tropical and subtropical regions of Asia, has drawn a lot of interest from the food technology sector due to its potential to…

The Effect of the Utilization of Lavender (Lavandula angustifolia) and Butter…

Currently, snacking has become a daily staple food as Indonesians consume more snacks compared to proper meals. Cereal bars have been well-known as a convenient snack that is easy to prepare and can be consumed anywhere. Given the current trend for cereal bars, ingredients that could produce a palatable cereal bar with sufficient nutritional content must be taken into account so the needs of…

The Effects of Baking Parameters on Sensory Properties of White bread Made wi…

White and sweet bread consumption in Indonesia grew to 3.01 kg/capita/year in 2020. This surge is due to bread's ease of preparation and consumption. Bread is a food product composed of wheat flour, water, sugar, salt, butter, and yeast. Yeast that is used to make bread is frequently dry yeast or instant yeast. Saccharomyces cerevisiae is the primary yeast in bread making. Natural yeast, de…

Effects of High-Pressure Processing on the Physicochemical, Microstructural, …

Negative effects on meat consumption have prompted the rise of protein alternatives. Although chicken is rich in protein, it has no dietary fibre. Hence, alternative fibre and protein-rich ingredients can be found in shiitake mushrooms with an umami flavour and tempeh with a dense texture. High-pressure processing (HPP) can improve the texture and technological functionalities of various foo…

Comparative Analysis of High-Pressure Processing Effects on Physicochemical, …

Excessive meat consumption's negative effects have driven the development of meat alternatives. While chicken is protein-rich, it lacks fiber, so mushrooms were used for their high fiber content, and jackfruit's fibrous texture to mimic chicken. High-pressure processing (HPP) improved texture and preserved nutritional value. This study aimed to develop nuggets using chicken, hybrid, and PBM…

The Effect Of Baking Parameters On Physicochemical properties Of White Bread …

Bread is widely favoured food due to simple composition and convenient suitability for daily consumption. One of the main ingredients used to determine the bread quality is yeast. As the Indonesian bread industry has grown, bread is being processed by replacing instant yeast with natural yeast because of increasing consumer interest natural products. Natural yeast is obtained from fermented…

Utilization of Snake Fruit (Salacca Zalacca) for Arak Using Distillers Active…

Arak, a traditional Indonesian spirit typically produced from rice or sugarcane, is made by fermenting rice or fruit juice followed by distillation. This research explores using snake fruit (Salacca Zalacca var Pondoh) as an innovative raw material for Arak production, offering a novel flavor profile and creating a niche market. Snake fruit, containing fermentable sugars, presents a cost-eff…

The effects of IFNγ, TNFα, and Dexamethasone onthe Immunopeptidome of Diffu…

Diffuse Intrinsic Pontine Glioma (DIPG) is a malignant tumor found in the brain stem which mainly affects children. It has an aggressive nature with no available curative treatment. Cancer immunotherapy using specific tumor antigens to target tumor cells may be a viable alternative therapeutic option for DIPG, however antigen presentation is highly downregulated in tumors. This study aims t…

Investigation of Gene Dependencies in Paediatric Tumour Models Using CRISPR-C…

Paediatric cancer accounts for the majority of cancer-related mortality in children. Although current treatment increased the overall survival rates, young individuals often encounter life-long side effects from toxic therapies. Therefore, there is an urgent need for targeted therapies aimed at tumour-specific vulnerabilities for greater specificity and reduced toxicity, in which precision m…

Optimizing Mycoprotein Production using Aspergillus oryzae in Soy Whey Substr…

Mycoproteins, that are produced through filamentous fungi fermentation, have gained popularity because of their rich protein content and sustainability as an animal-based protein alternative. However, the cost of the commerciallized mycoprotein exceeds the cost of regular meat. Therefore, scientists are exploring various inexpensive substrates and GRAS fungal strains with high survivability …

Investigating the Role of Tumor-Associated Neutrophils (TANs) in the Suppress…

Glioblastoma (GBM) is known as a highly aggressive and malignant form of brain cancer. Despite advances in understanding the genetic and molecular basis of glioblastoma, it has a poor prognosis with a median survival rate of 9 months. The complex interplay between malignant cells and the surrounding tumor microenvironment (TME) plays a pivotal role in tumor progression and treatment respons…