Hydroponic cultivation has emerged as a cost-effective alternative to traditional soil-based agriculture and may be less susceptible to pests and diseases. They can be set up in controlled environments, making them suitable for year-round production in urban areas with limited land availability. EM4 is a type of fertilizer that can increase agricultural systems' decomposition, composting, a…
Wound dressings play a crucial role in promoting efficient wound closure and healing by providing an environment conducive to tissue repair. Traditional wound dressings, such as cellulose-based gauze, often exhibit challenges in adhesion and exudate absorption, necessitating frequent replacements that may cause additional trauma. Biocellulose (BC), derived from bacteria, presents a natural c…
Bacterial cellulose (BC) is a biopolymer currently being coveted for its versatility, sustainable sourcing and biodegradability. The Indonesia International Institute for Life Sciences (i3L) lab has been conducting research on BC fermentation and surrounding BC produced by the kombucha isolate Komagataeibacter intermedius, with past findings showing a great prospect of the bacteria being abl…
The demand for fresh-cut fruits and vegetables (FFVs) has expanded due to consumer preferences for nutrition, safety, convenience, and ready-to-eat. However, FFVs are highly susceptible to surface browning and microbial spoilage. Active packaging (AP) offers a promising method to enhance shelf life and quality of FFVs. In this study, cellulose acetate-based active packaging films infused wi…
The gut microbiota represents a vast reservoir of bioactive substances with potential implications for human health and disease. This study focused on the identification of a novel metabolite originating from the gut microbiota strain Aneurinibacillus aneurinilyticus and evaluated its antibacterial properties. Advanced analytical techniques such as high-performance liquid chromatography, high-r…
The crisis of antibacterial resistance is an inevitable consequence of the widespread use of antibacterial agents in human health-related settings and agriculture. One of the oldest strategies to combat this crisis is to screen for novel antibacterial drug classes or structures, leading to the discovery of numerous antibacterial compounds, many of which are isolated from actinomyces/ Streptomyc…
Infantile spasm is a type of epilepsy that occurs in infants that is characterized by the sudden movement throughout the whole body. It is crucial to detect the disease and undergo suitable treatments as soon as possible in order to minimize the potential negative long-term effect in learning development. One of the most effective treatments is the usage of anti-epileptic drug Vigabatrin that i…
Soy sauce is a popular condiment in Asian countries made from fermented soybeans. The production process of traditional soy sauce usually takes place in a non-sterile environment, causing spontaneous fermentation by indigenous microorganisms. One of the commonly found and reported species in soybean-based fermented food was Bacillus subtilis. Although Bacillus is generally recognized as saf…
This study observed the physicochemical and microbiological stability of plant based meatballs made from local ingredients which are vacuum packed and frozen. During frozen storage, deterioration happened gradually caused by microbial growth and physicochemical changes. Thus, the microbial content including bacteria (TPC), yeast and mold (TYMC), and physicochemical aspects including water a…
Pediococcus acidilactici has gained a lot of interest lately due to its ability as a potential probiotic strain. However, concerns regarding its viability and stability during consumption and storage lead to a solution to utilize spray dry as an encapsulation method for Pediococcus acidilactici. Yet, the utilization of spray drying could affect the cell viability and its functional probiotic pr…
Preimplantation genetic testing (PGT) is a process to prioritise in vitro fertilised embryos without genetic defects for implantation. One of the conditions screened is aneuploidy. The genetic testing for chromosomal aneuploidy ensures that all 23 pairs of chromosomes have the correct copy number. The research utilized amplicon sequencing to generate sequence data and variant calling to p…
Southeast Asian Ovalocytosis (SAO) is a hereditary genetic disorder caused by a 27 bp deletion in the SLC4A1 gene, leading to a disrupted folding at the extracellular face of the SLC4A1 protein in the red blood cell (RBC) membrane. SAO is an autosomal dominant disorder where heterozygous individuals are asymptomatic and homozygous SAO are considered embryonic lethal. SAO can be observed microsc…
Brock Biology of Microorganisms continues to set the standard for impeccable scholarship, accuracy, and outstanding illustrations and photos. This book for biology, microbiology, and other science majors balances cutting edge research with the concepts essential for understanding the field of microbiology.
Human immunodeficiency virus 1 (HIV-1) has persistently been a global health concern, infecting 39 million people around the world with 1.3 million cases increment in 2020, leading to severe immunodeficiency (UNAIDS, 2023). While mammalian genomes show evidence of repeated infection with retroviral pathogens, this co-evolution has resulted in the emergence of defense mechanisms that pre…
"The study delves into the characterization of bacterial communities within Takakura composting. Takakura composting is a method integral to organic waste management, and serves as the focal point for understanding microbial dynamics during decomposition. The research aims to make takakura compost with two different compositions and characterize the bacterial community in the Takakura compo…
Tuak is an alcoholic beverage from Southeast Asian countries, such as Indonesia and Malaysia, prepared by fermenting starchy materials (e.g., rice, palm sap, coconut sap or water). Tuak production factors and recipes vary from one maker to another, including the type of substrate used, sugar level, water level, fermentation time, and even the starter culture used. These factors affect the micro…
This study highlights the environmental consequences of landfill buildup while addressing the issue of insufficient food waste management in Indonesia and the 1.3 billion yearly instances worldwide. The study focuses on the Takakura Composting Method (TCM), which combines fermentative microorganisms and locally created materials to provide a sustainable household-scale solution. The Takakur…
Diabetes is one of the most lethal and ninth most common diseases in the world, which is a metabolic disorder that causes a high and unusual blood sugar level due to the inability of the body to utilize sugar. Diabetes can cause multiple complications and damage to the system, one of them is diabetic foot ulcers (DFU). The most advanced treatment nowadays for DFU is by utilizing bio-cellulose, …
The gut microbiota determines the intestinal health status of the host by regulating gut epithelial and immune functions. Depletion of the beneficial gut microbiota after radiation exposure contributes to an exaggerated inflammatory response and dysregulation of intestinal epithelial cells. Previous studies demonstrated that administration of beneficial gut microbiota, Lactobacillus acidophilus…
Diospyros digyna is a wonderful fruit because of the fruit’s delicacy but not only that also because of how the fruit is easy to grow in most environments it can encounter and pest free mostly due to the effect of secondary metabolites called phytochemicals compounds. The study aimed to investigate the antibacterial properties of Unripe and ripe Diospyros digyna fruits against S. aureus and P…
Staphylococcus aureus is an opportunistic pathogen that commonly inhabits the human skin and can cause skin infection, especially in individuals with impaired immune systems when skin is damaged. Treatments usually involve the usage of antibiotics. However, antibiotic effectiveness is in danger due to the fast development of resistant microorganisms worldwide. As a result, there is a growing …
Folic acid is an important vitamin for fetal development and nucleotide and amino acid metabolism. Corn is one of the good sources for folic acid while tempeh fermentation has been reported to increase folic acid content. To concentrate further, membrane filtration is a promising technology. This study aims to understand the effect of microfiltration conditions towards the increase of folic aci…
The exploration of bacterial entry points into plants has been focused on natural openings such as stomata, lenticels, and plasmodesmata. However, the potential role of lateral root (LR) gaps as an entry portal for bacteria remains largely unexplored. This study attempts to investigate the correlation between lateral root number and bacterial colonization, with implications for human health thr…
Cancer is one of the deadliest diseases worldwide, with metastasis serving as the primary cause of death. While metastasis has been widely studied, a significant amount still remains unexplored, particularly the role of glycosylation. Glycosylation is a fundamental aspect in a variety of biological processes, indicating that slight changes in it can lead to detrimental effects such as cancer, w…
Polyethylene terephthalate (PET) and polyurethane (PU) are two examples of plastics that have integrated seamlessly into our daily lives. The qualities that make plastic so well-known are its low cost, light weight, and strength. However, plastics also can harm the environment when they are no longer in use because they are difficult to break down. The microbial plastic degrading method has …
Plastics such as polyethylene terephthalate (PET) and polyurethane (PU) are utilized extensively in all sectors of life due to their versatility, durability, and low cost. However, its poor degradability has led to plastic waste accumulation worldwide, making it a major source of pollution. Recently, there has been growing interest in microbial plastic degradation as a promising strategy to …
A preliminary antimicrobial assay showed the antimicrobial activity of P. acidilactici but the SDS-PAGE did not reveal pediocin, an antimicrobial peptide produced by Pediococcus strain. The efficient downstream processing of P. acidilactici fermentation is studied to analyze pediocin. This research is conducted to assess the compatibility of different methods in concentrating pediocin in th…
The deep-sea ecosystem is a largely unexplored environment with unique chemical and physical conditions that impact the organisms living there. Deep-sea sediments contain a diverse population of electroactive bacteria (EABs) that have the potential to generate electricity in microbial fuel cells (MFCs). This study aims to investigate the influence of salinity concentration on bacterial viabi…
The isolation and cultivation of indigenous bacteria poses a great importance for examining potential biodegradation microorganisms for a contaminated site. While the traditional method utilized for the isolation and cultivation method of bacteria has significant limitations and drawbacks, this study proposed the usage of a microwell array device that is able to isolate different bacteria a…
The increasing global electricity consumption driven by population growth and industrial development necessitates the exploration of environmentally friendly renewable energy sources. Microbial fuel cells (MFCs), more specifically deep-sea sediment microbial fuel cells, have gained significant attention in the bioenergy industry as they can harness bacteria capability to generate electricit…
The demand of a food preservative has been increasing due to foodborne diseases due to foodborne pathogens. Moreover, there is also an issue on the health risks caused chemical food preservatives, further increasing demand on a safer food preservative. Recently, Pediococcus acidilactici has been gaining interest as a potential natural food preservative due to its ability to produce pediocin,…
Environmental problems are increasing public awareness of the importance of protecting the environment. However, this awareness is not directly proportional to the buying behavior of green products. It is caused by several factors: lack of availability, high costs, lack of awareness, perceptions of lack of effectiveness, and greenwashing. Therefore, marketing green products, companies mus…
The utilization of edible film has gained much attention in food packaging development and industry due to its potential to preserve food and biodegradability, which could reduce environmental waste compared to conventional food packaging. Moreover, the application of pure polymers has shown insufficient defense against microbial deterioration; thus, the incorporation of plant-derived secon…
The demand of a food preservative has been increasing due to foodborne diseases due to foodborne pathogens. Moreover, there is also an issue on the health risks caused chemical food preservatives, further increasing demand on a safer food preservative. Recently, Pediococcus acidilactici has been gaining interest as a potential natural food preservative due to its ability to produce pediocin,…
Syzygium aromaticum, commonly known as cloves, is considered as one of the richest dietary sources of antioxidant and polyphenolic compounds. The bioactivity and antimicrobial activity of this spice is enhanced by using novel extraction techniques such as ultrasound-assisted extraction (UAE). Sample-to-solvent ratio is one of the important factors that impact the functional properties of pl…
This internship report focuses on the utilization of winnowing waste which was cacao husks into variants of cacao tea. Huge amounts of cacao waste are being thrown away, which causes environmental pollution in along with increasing economic issues. One of the waste products of chocolate that are typically discarded as waste are cacao husks. Cacao husks could be transformed into a variety o…
Black sapote is a fruit originating from Mexico that contains high antioxidant activity because of its varying phenolic compounds and micronutrients. Kombucha is one of the beverages that is fermented by a SCOBY to produce a unique and fizzy flavor. A SCOBY consists of acetic acid bacteria, lactic acid bacteria, and yeasts produce some acid for tart and fizzy flavour in kombucha. The effect …
This internship project aims to evaluate the microbial quality of coffee milk during its production at Jago Coffee. The project employs the Total Plate Count (TPC) and Methylene Blue Reduction Test (MBRT) to assess bacterial load and microbiological activity. The TPC results obtained for the cooling and filling & sealing processes comply with the SNI (Standar Nasional Indonesia) standards, …
Chronic diabec wound is a common complicaon in diabetes mellitus which is caused by a prolonged wound-healing process from a persistent inflammaon phase. Thus, proper wound treatment is needed to promote wound healing and protect the wound from infecon. Biocellulose (BC) as wound dressing provides a moist environment to protect skin and pr…
BIOS Instant Notes in Microbiology, Fourth Edition, is the perfect text for undergraduates looking for a concise introduction to the subject, or a study guide to use before examinations. Each topic begins with a summary of essential facts-an ideal revision checklist-followed by a description of the subject that focuses on core information, with clear, simple diagrams that are easy for students …
PT. Organik Adil Sejahtera (Green Rebel) is a branch company of Indonesia leading plant-based eatery chain, focused on providing demand from B2B and for ready-to-cook frozen meals. Responsible for products’ quality, QC/QA department monitors the process starting from incoming raw material inspection, base and product organoleptic testing, until complaint and CAPA filing. GMP audit and assisti…
Natural raw materials have been used for human health for centuries. Because of their distinct chemical composition and structure, wood and its bark are among the most important raw materials. The inner bark has a strong defense mechanism, which is supported by phenolic compounds and may contain a variety of antimicrobial agents that can be extracted and used as a food preservative. This study …
The increase of ornament plants always has a segmented market and unique economical value. Leatherleaf fern (Rumohra adiantiformis) was one of the examples of ornamental plants and was widely used in the floristry industry. Plant tissue culture has been an advance and conscientious alternative approach for plant cultivation to fulfill the commercial and market needs with a very mindful and …